Journal: BioTechniques

Article Title: Introduction of the hybcell-based compact sequencing technology and comparison to state-of-the-art methodologies for KRAS mutation detection.

doi: 10.2144/000114264

Figure Lengend Snippet: Figure 1. Schematic diagram of the hybcell-based compact sequencing technology. DNA is amplified and labeled using a common thermocycler followed by the introduction of the generated amplicons into the hybcell. (A) Labeled amplicons hybridize to the primers spotted on the surface of the hybcell. (B) Only those DNA molecules with a perfect match in KRAS codons 12 and 13 are extended. (C) The others are removed after a stringent wash, and the signal of the labeled and bound mutated DNA molecules is then measured.

Article Snippet: Sensitive sequencing method for KRAS mutation detection by Pyrosequencing.

Techniques: Sequencing, Amplification, Labeling, Generated

Journal: BioTechniques

Article Title: Introduction of the hybcell-based compact sequencing technology and comparison to state-of-the-art methodologies for KRAS mutation detection.

doi: 10.2144/000114264

Figure Lengend Snippet: Figure 2. Overview of the workflow and turnaround time of 454-NGS (GS FLX+), Sanger sequencing (Genetic Analyzer 3130xL), pyrosequencing (PyroMark Q24), and hybcell-based compact sequencing.

Article Snippet: Sensitive sequencing method for KRAS mutation detection by Pyrosequencing.

Techniques: Sequencing